Differential glycosylation of the CD8a homodimer regulates binding to the non-classical MHC-I, H2-Q10 — ASN Events
  1. Schedule
  2. Poster Session One & Burnet Oration Cocktail Function
  3. Differential glycosylation of the CD8a homodimer regulates binding to the non-classical MHC-I, H2-Q10

Differential glycosylation of the CD8a homodimer regulates binding to the non-classical MHC-I, H2-Q10 (#226)

Katharine J Goodall 1 , Angela Nguyen 1 , Aya Matsumoto 2 , Sneha Sant 3 , Katherine Kedzierska 3 , Julie McMullen 2 , Joseph Trapani 4 , Mariapia Degli-Esposti 5 , Jamie Rossjohn 6 7 8 9 , Lucy C Sullivan 10 , Dan M Andrews 1
  1. Immunology and Pathology, Monash University, Melbourne, Victoria, Australia
  2. Baker Heart & Diabetes Institute, Melbourne, Victoria, Australia
  3. Immunology, Peter Doherty Institute, Melbourne, Victoria, Australia
  4. Cancer Immunology, Peter MacCallum Cancer Centre, Melbourne, Victoria, Australia
  5. Lions Eye Institute, Perth, Western Australia, Melbourne
  6. Biochemistry and Molecular Biology, Monash University, Melbourne, Victoria, Australia
  7. Australian Research Council Centre of Excellence in Advanced Molecular Imaging, Monash University, Melbourne, Victoria, Australia
  8. Infection and Immunity Program, Biomedicine Discovery Institute, Melbourne, Victoria, Australia
  9. Institute of Infection and Immunity, Cardiff University School of Medicine, Cardiff, Wales
  10. Microbiology and Immunology, Peter Doherty Institute, Melbourne, Victoria, Australia

Gamma delta T cells are conserved across ~500 million years of evolution, highlighting their importance to immunity. Despite this, the identification of receptor ligand interactions that control their development has not been largely explored. In this study we demonstrate that the class Ib MHC, H2-Q10, binds to the CD8αα homodimer found on gamma delta T cells. The importance of this interaction is underscored by our observation that recognition of H2-Q10 is controlled by differential glycosylation between gamma delta and alpha beta T cells. We also demonstrate that H2-Q10 and CD8αα expressing gamma delta T cells are resident in the liver and their interaction is important to cellular development. These results provide the first evidence that glycosylation is differentially regulated amongst gamma delta and alpha beta T cells. Furthermore, they also suggest that co-evolution between class Ib MHC and gamma delta T cells may play an important role in their biology.

#ASI2017QLD