Systemic Lupus Erythematosus (SLE) is an autoimmune inflammatory disorder that could affect any organ system in the body. SLE is a potentially destructive disease with the involvement of all the components of the immune system. The aim of this study was to decode the expression of cytokines, chemokines and growth factors in the sera of SLE patients, using magnetic multiplex immunoassay method, for devising effective therapies. Serum was isolated from SLE patients (n = 28) at the Rheumatology Clinic, King Abdulaziz University Hospital (KAUH), Jeddah, Kingdom of Saudi Arabia (KSA) after obtaining written informed consent. Serum of normal controls (n = 12) and osteoarthritis (OA) patients (n=9) were also obtained. Human Cytokine Magnetic 30-plex panel (ThermoFisher Scientific, USA) was used to get the differential expression pattern of the cytokines, chemokines and growth factors by MAGPIX (Luminex Corporation, USA). Also, the correlation of the 30-plex data with clinical parameters was accomplished using IBM SPSS Statistics version 22 (IBM Corporation, USA). Here, cytokines like TNFα, IL-1RA, IL-2, IL-2R, IL-5, IL-6, IFNγ, IFNα, IL-12, IL-13, and IL-4 were significantly increased (P<0.05) in SLE compared to healthy and OA groups. Chemokines, such as RANTES, IL-8, IP-10, MIG, MCP-1, Eotaxin, and growth factors such as G-CSF, and HGF were also significantly (P<0.05) increased in SLE. Moreover, the multiplex cytokine data from SLE patients showed a positive correlation with disease severity and other related clinical factors. Thus, the MAGPIX analysis of serum cytokines, chemokines and growth factors, is a reliable methodology to monitor the disease state accurately and, therefore, helps to formulate better therapies for SLE patients.